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igg2c fitc antibody  (SouthernBiotech)


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    SouthernBiotech igg2c fitc antibody
    ( A and B ) Serum anti-VLP antibody titers in control and BCAP KO mice immunized with 2 μg of VLPs containing ssRNA measured at 7, 14, 21, 28, and 35 days after immunization. ( C ) Fluorescence-activated cell sorting (FACS) plots of VLP-immunized control and BCAP KO mice splenocytes stained with fluorescent VLPs, showing VLP + cells in the B220 + gate. FSC-W, forward scatter width. ( D ) Proportion and count of spleen VLP + B cells [gated as in (C)] from control and KO mice immunized with 2 μg of VLPs for 35 days. ( E ) B220 + VLP + GC B cells were identified as Fas + CD38 − cells in control and KO mice. ( F ) Proportion and count of spleen VLP-specific GC B cells [gated as in (E)] from control and KO mice. ( G to I ) Splenocytes were gated as B220 + VLP + Fas − CD38 + non-GC B cells, and the frequencies and count of switched memory and IgM memory B cells were determined on the basis of IgM and IgD levels. ( J ) VLP-specific plasma cells of IgG or <t>IgG2c</t> isotype enumerated by ELISpot assay on bone marrow (BM) cells from control or KO mice harvested at day 35 after immunization. All data are from one of the two independent experiments yielding similar conclusions. Points represent individual mice ( n = 7 mice per group) with mean [(A) and (B)] or mean with SEM [(D) to (J)] shown. P values of less than 0.05 are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, calculated with two-way analysis of variance (ANOVA) with multiple comparisons test [(A) and (B)] or Mann-Whitney U test [(D) to (J)]. A.U., arbitrary units.
    Igg2c Fitc Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2c fitc antibody/product/SouthernBiotech
    Average 90 stars, based on 1 article reviews
    igg2c fitc antibody - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "B cell adapter for PI 3-kinase (BCAP) coordinates antigen internalization and trafficking through the B cell receptor"

    Article Title: B cell adapter for PI 3-kinase (BCAP) coordinates antigen internalization and trafficking through the B cell receptor

    Journal: Science Advances

    doi: 10.1126/sciadv.adp1747

    ( A and B ) Serum anti-VLP antibody titers in control and BCAP KO mice immunized with 2 μg of VLPs containing ssRNA measured at 7, 14, 21, 28, and 35 days after immunization. ( C ) Fluorescence-activated cell sorting (FACS) plots of VLP-immunized control and BCAP KO mice splenocytes stained with fluorescent VLPs, showing VLP + cells in the B220 + gate. FSC-W, forward scatter width. ( D ) Proportion and count of spleen VLP + B cells [gated as in (C)] from control and KO mice immunized with 2 μg of VLPs for 35 days. ( E ) B220 + VLP + GC B cells were identified as Fas + CD38 − cells in control and KO mice. ( F ) Proportion and count of spleen VLP-specific GC B cells [gated as in (E)] from control and KO mice. ( G to I ) Splenocytes were gated as B220 + VLP + Fas − CD38 + non-GC B cells, and the frequencies and count of switched memory and IgM memory B cells were determined on the basis of IgM and IgD levels. ( J ) VLP-specific plasma cells of IgG or IgG2c isotype enumerated by ELISpot assay on bone marrow (BM) cells from control or KO mice harvested at day 35 after immunization. All data are from one of the two independent experiments yielding similar conclusions. Points represent individual mice ( n = 7 mice per group) with mean [(A) and (B)] or mean with SEM [(D) to (J)] shown. P values of less than 0.05 are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, calculated with two-way analysis of variance (ANOVA) with multiple comparisons test [(A) and (B)] or Mann-Whitney U test [(D) to (J)]. A.U., arbitrary units.
    Figure Legend Snippet: ( A and B ) Serum anti-VLP antibody titers in control and BCAP KO mice immunized with 2 μg of VLPs containing ssRNA measured at 7, 14, 21, 28, and 35 days after immunization. ( C ) Fluorescence-activated cell sorting (FACS) plots of VLP-immunized control and BCAP KO mice splenocytes stained with fluorescent VLPs, showing VLP + cells in the B220 + gate. FSC-W, forward scatter width. ( D ) Proportion and count of spleen VLP + B cells [gated as in (C)] from control and KO mice immunized with 2 μg of VLPs for 35 days. ( E ) B220 + VLP + GC B cells were identified as Fas + CD38 − cells in control and KO mice. ( F ) Proportion and count of spleen VLP-specific GC B cells [gated as in (E)] from control and KO mice. ( G to I ) Splenocytes were gated as B220 + VLP + Fas − CD38 + non-GC B cells, and the frequencies and count of switched memory and IgM memory B cells were determined on the basis of IgM and IgD levels. ( J ) VLP-specific plasma cells of IgG or IgG2c isotype enumerated by ELISpot assay on bone marrow (BM) cells from control or KO mice harvested at day 35 after immunization. All data are from one of the two independent experiments yielding similar conclusions. Points represent individual mice ( n = 7 mice per group) with mean [(A) and (B)] or mean with SEM [(D) to (J)] shown. P values of less than 0.05 are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, calculated with two-way analysis of variance (ANOVA) with multiple comparisons test [(A) and (B)] or Mann-Whitney U test [(D) to (J)]. A.U., arbitrary units.

    Techniques Used: Control, Fluorescence, FACS, Staining, Clinical Proteomics, Enzyme-linked Immunospot, MANN-WHITNEY



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    ( A and B ) Serum anti-VLP antibody titers in control and BCAP KO mice immunized with 2 μg of VLPs containing ssRNA measured at 7, 14, 21, 28, and 35 days after immunization. ( C ) Fluorescence-activated cell sorting (FACS) plots of VLP-immunized control and BCAP KO mice splenocytes stained with fluorescent VLPs, showing VLP + cells in the B220 + gate. FSC-W, forward scatter width. ( D ) Proportion and count of spleen VLP + B cells [gated as in (C)] from control and KO mice immunized with 2 μg of VLPs for 35 days. ( E ) B220 + VLP + GC B cells were identified as Fas + CD38 − cells in control and KO mice. ( F ) Proportion and count of spleen VLP-specific GC B cells [gated as in (E)] from control and KO mice. ( G to I ) Splenocytes were gated as B220 + VLP + Fas − CD38 + non-GC B cells, and the frequencies and count of switched memory and IgM memory B cells were determined on the basis of IgM and IgD levels. ( J ) VLP-specific plasma cells of IgG or <t>IgG2c</t> isotype enumerated by ELISpot assay on bone marrow (BM) cells from control or KO mice harvested at day 35 after immunization. All data are from one of the two independent experiments yielding similar conclusions. Points represent individual mice ( n = 7 mice per group) with mean [(A) and (B)] or mean with SEM [(D) to (J)] shown. P values of less than 0.05 are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, calculated with two-way analysis of variance (ANOVA) with multiple comparisons test [(A) and (B)] or Mann-Whitney U test [(D) to (J)]. A.U., arbitrary units.
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    Image Search Results


    ( A and B ) Serum anti-VLP antibody titers in control and BCAP KO mice immunized with 2 μg of VLPs containing ssRNA measured at 7, 14, 21, 28, and 35 days after immunization. ( C ) Fluorescence-activated cell sorting (FACS) plots of VLP-immunized control and BCAP KO mice splenocytes stained with fluorescent VLPs, showing VLP + cells in the B220 + gate. FSC-W, forward scatter width. ( D ) Proportion and count of spleen VLP + B cells [gated as in (C)] from control and KO mice immunized with 2 μg of VLPs for 35 days. ( E ) B220 + VLP + GC B cells were identified as Fas + CD38 − cells in control and KO mice. ( F ) Proportion and count of spleen VLP-specific GC B cells [gated as in (E)] from control and KO mice. ( G to I ) Splenocytes were gated as B220 + VLP + Fas − CD38 + non-GC B cells, and the frequencies and count of switched memory and IgM memory B cells were determined on the basis of IgM and IgD levels. ( J ) VLP-specific plasma cells of IgG or IgG2c isotype enumerated by ELISpot assay on bone marrow (BM) cells from control or KO mice harvested at day 35 after immunization. All data are from one of the two independent experiments yielding similar conclusions. Points represent individual mice ( n = 7 mice per group) with mean [(A) and (B)] or mean with SEM [(D) to (J)] shown. P values of less than 0.05 are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, calculated with two-way analysis of variance (ANOVA) with multiple comparisons test [(A) and (B)] or Mann-Whitney U test [(D) to (J)]. A.U., arbitrary units.

    Journal: Science Advances

    Article Title: B cell adapter for PI 3-kinase (BCAP) coordinates antigen internalization and trafficking through the B cell receptor

    doi: 10.1126/sciadv.adp1747

    Figure Lengend Snippet: ( A and B ) Serum anti-VLP antibody titers in control and BCAP KO mice immunized with 2 μg of VLPs containing ssRNA measured at 7, 14, 21, 28, and 35 days after immunization. ( C ) Fluorescence-activated cell sorting (FACS) plots of VLP-immunized control and BCAP KO mice splenocytes stained with fluorescent VLPs, showing VLP + cells in the B220 + gate. FSC-W, forward scatter width. ( D ) Proportion and count of spleen VLP + B cells [gated as in (C)] from control and KO mice immunized with 2 μg of VLPs for 35 days. ( E ) B220 + VLP + GC B cells were identified as Fas + CD38 − cells in control and KO mice. ( F ) Proportion and count of spleen VLP-specific GC B cells [gated as in (E)] from control and KO mice. ( G to I ) Splenocytes were gated as B220 + VLP + Fas − CD38 + non-GC B cells, and the frequencies and count of switched memory and IgM memory B cells were determined on the basis of IgM and IgD levels. ( J ) VLP-specific plasma cells of IgG or IgG2c isotype enumerated by ELISpot assay on bone marrow (BM) cells from control or KO mice harvested at day 35 after immunization. All data are from one of the two independent experiments yielding similar conclusions. Points represent individual mice ( n = 7 mice per group) with mean [(A) and (B)] or mean with SEM [(D) to (J)] shown. P values of less than 0.05 are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, calculated with two-way analysis of variance (ANOVA) with multiple comparisons test [(A) and (B)] or Mann-Whitney U test [(D) to (J)]. A.U., arbitrary units.

    Article Snippet: IgG2c (FITC, catalog no. 1079-02) and anti-mouse IgM biotinylated (catalog no. 1021-08) were purchased from SouthernBiotech.

    Techniques: Control, Fluorescence, FACS, Staining, Clinical Proteomics, Enzyme-linked Immunospot, MANN-WHITNEY

    List of immunophenotypic markers or gene IDs, alternative names, and their descriptions

    Journal: iScience

    Article Title: Nanosphere pharmacodynamics improves safety of immunostimulatory cytokine therapy

    doi: 10.1016/j.isci.2024.108836

    Figure Lengend Snippet: List of immunophenotypic markers or gene IDs, alternative names, and their descriptions

    Article Snippet: CF594 CEACAM8 antibody, polyclonal , Biorbyt , orb213728-CF594.

    Techniques: Virus, Marker, Immunopeptidomics, Binding Assay

    Journal: iScience

    Article Title: Nanosphere pharmacodynamics improves safety of immunostimulatory cytokine therapy

    doi: 10.1016/j.isci.2024.108836

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    Article Snippet: CF594 CEACAM8 antibody, polyclonal , Biorbyt , orb213728-CF594.

    Techniques: Recombinant, Staining, Cell Culture, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Isolation, Multiplex Assay, Software, Sterility